Phosphodiesterase 3 inhibitors selectively block the spontaneous resumption of meiosis by macaque oocytes in vitro
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منابع مشابه
Effect of Fibroblastic Growth Factor on Resumption of Meiosis, In Vitro Maturation And Embryo Development of Immature Mouse Oocytes
Purpose: The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without basic fibroblastic growth factor-4 (bFGF-4). Materials and Methods: cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after adm...
متن کاملDose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes
Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was designed to determine the effect of Gundelia Tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: In this stu...
متن کاملDose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes
Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was designed to determine the effect of Gundelia Tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: In this stu...
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The calcium-binding protein, calmodulin, has been purified from Xenopus laevis oocytes. This 18,500-dalton protein, pl 4.3, has two high-affinity calcium-binding sites per mole protein having a dissociation constant of 2.8 x 10(-6) M. Full-grown Xenopus oocytes, arrested in late G2 of the meiotic cell cycle, resumed meiosis when microinjected with 60-80 ng (3-4 pmol) of calmodulin in the form o...
متن کاملThe effect of cdk kinase inhibitors on resumption of meiosis and activation of porcine oocytes
Butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases, was shown to block germinal vesicle breakdown (GVBD) in porcine oocytes in a concentration-dependent manner. GVBD was almost totally inhibited over the course of 44 h of culture when 100 pM BL I was included in TCM 199 containing either 3 mg/ml BSA or 5 % fetal calf serum (FCS). In contrast, 50 pM BL 1 was full...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2002
ISSN: 1460-2350
DOI: 10.1093/humrep/17.8.2079